Riboswitches and RNA thermometers (RNATs) are regulatory elements contained of many bacterial riboswitches have deciphered the molecular architecture of .. to complete translation of downstream gene in a zipper like fashion [70]. Fig. Bacterial RNA thermometers: molecular zippers and switches (English). 0 references. author name string. Jens Kortmann. series ordinal. 1. 3 Catalytic RNAs RNA binds metal ions that function Kortmann J, Narberhaus F. Bacterial RNA thermometers: molecular zippers and switches.

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Transient RNA structure features are evolutionarily conserved and can be computationally predicted. However, while computational methods are advanced enough to accurately predict short and stable secondary structures, their reliability decreases substantially with increasing length of the RNA molecule or when complex structures, such as pseudoknots and other tertiary interactions, come into play.

Temperature triggers immune evasion by Neisseria meningitidis. Not only the sequence but also the overall architecture differs substantially among presently known RNAT.

Heat-induced synthesis of sigma32 in Escherichia coli: This structure is much weaker in the mutated RNA. Genome-wide bioinformatic prediction and experimental evaluation of potential RNA thermometers. After DMS treatment, the RNA is isolated and the modification position is detected by reverse transcription and deep sequencing.


Together, in vivo and in vitro data provide valuable complementary information to unveil biologically relevant structures and their dynamics. Hydroxyl radicals cleave at RNA bases that are solvent-exposed. Secondary structure of the ribosome binding site determines translational efficiency: Temperature-controlled structural alterations of an RNA thermometer. Direct observation of the temperature-induced melting process of the Salmonella fourU RNA thermometer at base-pair resolution.

This approach permits the identification of ss regions only. Comparison between in vitro and in vivo data will ultimately provide a detailed picture of the RNA structurome in its physiological context. Base pairing of proximal nucleotides generates secondary structures, like stem-loops.

Bacterial RNA thermometers: molecular zippers and switches.

Although often successful, this approach is laborious because every RNAT candidate must be experimentally validated, for example by reporter gene fusions, typically in E. Topics Discussed in This Paper. NMIA attacks flexible unpaired bases and forms 2-O adducts that terminate reverse transcription.

Nature and contributions to pathogenesis Jameel M. Anabaena variabilis encodes two sHSPs. RNA thermometer-mediated translational regulation. The prfA thermosensor is peculiar as it integrates not only the temperature signal by structural changes but also metabolic information via a riboswitch-derived small regulatory RNA Loh et al.

Liberation of the RBS permits formation of the translation initiation complex and translation occurs.

Structural data can be used to constrain RNA structure prediction algorithms in order to obtain more accurate experimentally-derived secondary structure models of molefular the sequenced transcripts. Genome-wide measurement of RNA secondary structure in yeast. Global analysis of RNA secondary structure in two metazoans.


The prfA gene encodes a transcription factor that controls the synthesis of a number of important virulence factors.

Bacterial RNA thermometers: molecular zippers and switches – Semantic Scholar

Use of dimethyl sulfate to probe RNA structure in vivo. Other members of this class control synthesis of the periplasmic protease HtrA in Salmonella Klinkert et al. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Citations Publications citing this paper. This approach could successfully confirm single-strand regions of non-coding RNA with known structure and unveiled the secondary structure of non-coding RNA with previously unknown structure Underwood et al.

Only recently high-throughput sequencing technologies have been successfully applied to RNA structure probing in order to obtain experimentally-derived genome-wide insights into RNA folding. This paper has been referenced on Twitter 7 times over the past 90 days.